Volume in circulation
Useful Life
Annual capital prod.
Anticipated deprec.
Applied Biosystems StepOne 48-Well Real-Time
$28,000
(New) factory MSRP
introduced in 2009
Buy at:
$3,185.00
equilibrium price ; fetched May 15, 2025
Sell in 30 days for $1,911.00 on Westrn
Delivers with UPS between May 25, 2025 - May 30, 2025
About the Applied Biosystems StepOne 48-Well Real-Time
Description:
The StepOne brought factory-calibrated, dye-flexible real-time PCR to low-throughput molecular labs that still need publication-grade data. Its aluminum block accepts a single 48-well 0.1 mL plate and is driven by a Peltier stack that ramps at 4.6 °F s⁻¹ (standard) or 8.3 °F s⁻¹ (fast mode), completing a 40-cycle assay in as little as 40 minutes. Three narrow-band LEDs excite FAM/SYBR Green, VIC/JOE/HEX and ROX/reference channels; emission is split to a cooled photodiode array that quantifies each well every cycle, delivering nine-log linearity and single-copy sensitivity. Because the optical paths are pre-aligned and gain-balanced at the factory, users can swap plasticware or dyes without manual calibration. The front USB slot runs the system PC-free with a flash drive, yet Ethernet lets larger facilities push data straight to a LIMS. A silent internal fan and 9.7-in footprint suit teaching benches, while front hot-swap filters future-proof the rig for new fluorophores.
Economic Advantage:
By limiting capacity to 48 wells, the StepOne sells for less than half the price of 96-well instruments yet retains the same ramp rates, optics and software—ideal for core labs that bill by plate rather than sample. Fast cycling shaves two hours off a typical day’s work, reclaiming labor worth well over the differential to a block thermocycler within the first year.
Price History
Specifications:
| Capacity | 48 × 0.1 mL tubes / 1 plate |
|---|---|
| Temp. range | 39 °F – 212 °F (4 – 100 °C) |
| Peak block rate | 4.6 °F s⁻¹ (2.6 °C s⁻¹) |
| Fast ramp | 8.3 °F s⁻¹ (4.6 °C s⁻¹) |
| Thermal accuracy | ±0.45 °F (0.25 °C) |
| Uniformity | ±0.45 °F (0.25 °C) |
| Run time | < 40 min (fast); < 2 h (standard) |
| Optical channels | FAM/SYBR, VIC/HEX, ROX reference |
| Dynamic range | ≥ 9 log units |
| Sensitivity | 1-copy detection |
| Power | 100–240 V AC, 4 A |
Included Accessories:
You order with Westrn will include:
- (1) Hospital grade power cord
Unless, indicated otherwise, or in cases where accessories are single use disposable, these accessories will arrive in "pre-owned" condition.
Sales Data
Includes all sales of the Applied Biosystems StepOne 48-Well Real-Time within the last 12 months on the Westrn platform. Amounts do not include taxes or fees found in checkout subtotal.
No sales history available.